Journal: Frontiers in Genetics

Article Title: WTAP-mediated m6A methylation of SOX2 affects lung adenocarcinoma malignancy via Wnt/β-catenin pathway

doi: 10.3389/fgene.2026.1756241

Figure Lengend Snippet: Upregulation of SOX2 in LUAD. (A) Differential expression of SOX2 (log2 (TPM+1)) in LUAD was analyzed using the TCGA-LUAD dataset; (B) qRT-PCR was conducted to assess the mRNA expression levels of SOX2 in LUAD tissues and adjacent normal tissues; (C) Western blotting was conducted to assess the protein expression levels of SOX2 in LUAD tissues and adjacent normal tissues; (D) The mRNA expression of SOX2 was measured in human normal bronchial epithelial cells (BEAS-2B) and LUAD cell lines (A549, HCC827) by qRT-PCR. (E) The protein expression of SOX2 was measured in human normal bronchial epithelial cells (BEAS-2B) and LUAD cell lines (A549, HCC827) by Western blotting. * P < 0.05.

Article Snippet: To address this gap, experiments were conducted by knocking down SOX2 and treating cells with the Wnt pathway activator Wnt/β-catenin agonist 3 (MCE, USA).

Techniques: Quantitative Proteomics, Quantitative RT-PCR, Expressing, Western Blot

Journal: Frontiers in Genetics

Article Title: WTAP-mediated m6A methylation of SOX2 affects lung adenocarcinoma malignancy via Wnt/β-catenin pathway

doi: 10.3389/fgene.2026.1756241

Figure Lengend Snippet: SOX2 knockdown attenuates LUAD malignancy. A549 cells were stratified into two groups: si-NC and si-SOX2. (A) Transfection efficiency was confirmed by qRT-PCR in each group; (B) Western blotting was used to detect the protein expression of SOX2. (C) Colony formation assays were used to evaluate cell proliferation in each group; (D) Migration and invasion were analyzed using Transwell assays in each group; (E) Apoptosis was detected by flow cytometry in each group. (F) Cell cycle was detected by flow cytometry in each group. * P < 0.05.

Article Snippet: To address this gap, experiments were conducted by knocking down SOX2 and treating cells with the Wnt pathway activator Wnt/β-catenin agonist 3 (MCE, USA).

Techniques: Knockdown, Transfection, Quantitative RT-PCR, Western Blot, Expressing, Migration, Flow Cytometry

Journal: Frontiers in Genetics

Article Title: WTAP-mediated m6A methylation of SOX2 affects lung adenocarcinoma malignancy via Wnt/β-catenin pathway

doi: 10.3389/fgene.2026.1756241

Figure Lengend Snippet: SOX2 affects LUAD progression through the Wnt/β-catenin pathway. A549 cells were grouped into four categories: si-NC + DMSO, si-SOX2 + DMSO, and si-SOX2 + Wnt/β-catenin agonist 3. (A) Western blotting was used to detect the protein expression of SOX2, β-catenin, and c-Myc; (B,C) : Proliferation was evaluated by colony formation assays in each group; (D) Migration and invasion were analyzed using Transwell in each group; (E) Apoptosis was detected by flow cytometry in each group. (F) Cell cycle was detected by flow cytometry in each group. * P < 0.05.

Article Snippet: To address this gap, experiments were conducted by knocking down SOX2 and treating cells with the Wnt pathway activator Wnt/β-catenin agonist 3 (MCE, USA).

Techniques: Western Blot, Expressing, Migration, Flow Cytometry

Journal: Frontiers in Genetics

Article Title: WTAP-mediated m6A methylation of SOX2 affects lung adenocarcinoma malignancy via Wnt/β-catenin pathway

doi: 10.3389/fgene.2026.1756241

Figure Lengend Snippet: WTAP-mediated m 6 A methylation promotes SOX2 mRNA decay. (A) m 6 A modification sites of SOX2 targeted by WTAP were predicted using bioinformatics tools; (B) Differential expression analysis of WTAP (log2 (TPM+1)) in LUAD was conducted based on the TCGA-LUAD dataset; (C) qRT-PCR was used to measure the mRNA expression levels of WTAP in human normal lung epithelial cells (BEAS-2B) and LUAD cells (A549, HCC827). (D,E) Western blotting was used to measure the protein expression levels of WTAP in human normal lung epithelial cells (BEAS-2B) and LUAD cells (A549, HCC827). A549 cells were assigned to two groups: oe-NC and oe-WTAP. (F) The mRNA expression of WTAP in each group was detected by qRT-PCR; (G) The mRNA expression levels of SOX2 in each group were measured by qRT-PCR; (H) Western blotting was used to measure the protein expression levels of SOX2. (I) m 6 A methylation levels in each group were detected by dot-blot assays; (J) The mRNA expression of SOX2 in each group was detected by MeRIP; (K) The mRNA expression of SOX2 in each group was detected by RIP; (L) The mRNA stability of SOX2 in each group was assessed by qRT-PCR. * P < 0.05.

Article Snippet: To address this gap, experiments were conducted by knocking down SOX2 and treating cells with the Wnt pathway activator Wnt/β-catenin agonist 3 (MCE, USA).

Techniques: Methylation, Modification, Quantitative Proteomics, Quantitative RT-PCR, Expressing, Western Blot, Dot Blot

Journal: Frontiers in Genetics

Article Title: WTAP-mediated m6A methylation of SOX2 affects lung adenocarcinoma malignancy via Wnt/β-catenin pathway

doi: 10.3389/fgene.2026.1756241

Figure Lengend Snippet: WTAP affects the malignant progression of LUAD through the SOX2-mediated Wnt/β-catenin pathway. A549 cells were grouped into three categories: oe-NC + oe-NC, oe-NC + oe-SOX2, and oe-WTAP + oe-SOX2. (A) Western blotting was used to detect the protein expression levels of SOX2, β-catenin, and c-Myc in each group; (B,C) : Colony formation assays were performed to evaluate the proliferative capacity of each group; (D) Migration and invasion abilities were analyzed using Transwell assays in each group; (E) Apoptosis was detected by flow cytometry in each group. (F) Cell cycle was detected by flow cytometry in each group. * P < 0.05.

Article Snippet: To address this gap, experiments were conducted by knocking down SOX2 and treating cells with the Wnt pathway activator Wnt/β-catenin agonist 3 (MCE, USA).

Techniques: Western Blot, Expressing, Migration, Flow Cytometry